Server for Multiple Structure-Guided Sequence Alignment of Protein Families

Opening soon ...

Need a multiple alignment of protein families ...

... to study protein function and structure,
annotate binding sites, identify and classify new family members ...

... ?

... by implementing a combination of bioinformatic techniques
to collect and align sequences and structures available in public databases

Want to get straight to the point with Mustguseal?
Step 1: Press the green button "Mustguseal it NOW!" above
Step 2: Scroll down, check out the "Algorithm outline" on the right side of the page
Step 3: Press the blue button "Demo mode"

What is Mustguseal?
Mustguseal stands for Multiple Structure-Guided Sequence Alignment.
Mustguseal is a bioinformatic protocol - designed to build alignments of protein families and superfamilies, and a platform - a server implementing the original and third-party algorithms to provide a user-friendly web-based interface to the Mustguseal protocol through the World Wide Web.

What does Mustguseal do?
Mustguseal protocol uses structure similarity search to collect remote evolutionary relatives, which are expected to represent different protein families. Then Mustguseal uses sequence similarity search to collect close evolutionary relatives - members of these families. In such a way Mustguseal takes into account complex structural and functional variability within a large superfamily to obtain a diverse set of homologous proteins. A combination of structure and sequence alignment procedures is then implemented to build the final multiple alignment.

What software is required to use Mustguseal?
All steps of the Mustguseal protocol are executed entirely on the server side. You do not need any specific software on your side.
The Analysis section of the Results page offers interactive content for sequence and structure analysis. Interactivity is implemented in HTML5 and therefore no plugins nor Java are required. The only prerequisite for viewing the Analysis section of the Results page is a HTML5-compatible web-browser. Current versions of all major browsers support HTML5 as the default standard.

What web-browser is required to use Mustguseal?
You do need a regular web-browser to use Mustguseal. To ensure the HTML5-compatibility upgrade your current browser to the latest version.
The server has been tested with the following browsers:
Google Chrome (;
Mozilla Firefox (;
Konqueror (
Please note that we have had compatibility issues with the Microsoft Internet Explorer and therefore this browser is currently not supported by the Mustguseal.