Server for Multiple Structure-Guided Sequence Alignment of Protein Families

Mustguseal can automatically construct large structure-based
sequence alignments of protein families that include thousands of proteins
basing on all available information about their structures and sequences in public databases

to help at studying a protein function and regulation, designing improved enzyme variants
for practical application and selective modulators of enzyme functional properties

Alternatively, for small monitors use [The version for small monitors]

Server version A 1.0; June 19th 2017

Want to get straight to the point with Mustguseal?

Step 1: Press the green button "Mustguseal it NOW!" above
Step 2: Scroll down, check out the "Algorithm outline" on the right side of the page
Step 3: Press the blue button "Demo mode"

Site Navigation:

The Mustguseal FAQs:

What is Mustguseal?
Mustguseal stands for Multiple Structure-Guided Sequence Alignment.
Mustguseal is a bioinformatic protocol designed to build alignments of protein families and superfamilies, and a web-platform to provide a user-friendly web-based interface to the Mustguseal protocol through the World Wide Web. Multiple alignments of thousands of protein sequences and structures can be automatically constructed using this public web-server. You should see the Presentation of the Mustguseal server for more information.

What does Mustguseal do?
The Mustguseal protocol implements structure similarity search to collect remote evolutionary relatives, which are expected to represent different protein families. Then, for each collected remote evolutionary relative, Mustguseal runs sequence similarity search to collect close evolutionary relatives - members of the corresponding families. In such a way Mustguseal takes into account complex structural and functional variability within a large superfamily to obtain a diverse set of homologous proteins. A combination of structure and sequence alignment procedures is then implemented to build the final multiple alignment. Multiple alignments of thousands of protein sequences and structures can be automatically constructed using this public web-server.

What software is required to use Mustguseal?
All steps of the Mustguseal protocol are executed entirely on the server side. You do not need any specific software on your side.
The Analysis section of the Results page offers interactive content for sequence and structure analysis. Interactivity is implemented in HTML5 and therefore no plugins nor Java are required. The only prerequisite for viewing the Analysis section of the Results page is a HTML5-compatible web-browser. Current versions of all major browsers support HTML5 as the default standard.

What web-browser is required to use Mustguseal?
You do need a regular web-browser to use Mustguseal. To ensure the HTML5-compatibility upgrade your current browser to the latest version.

The server has been successfully tested for compatibility with the following browsers:

Please note that we have had compatibility issues with the Microsoft Internet Explorer and therefore this browser is currently not supported by the Mustguseal.

How to cite Mustguseal?
See this page for details.

How can a Mustguseal alignment help in your research?
You should use sister services of Mustguseal to further study the obtained alignments:

  • the Zebra web-service to identify variable amino acid residues responsible for functional diversity and to select hotspots for directed evolution or rational design experiments;

  • the pocketZebra web-service to identify and rank binding sites in proteins by functional significance and select particular positions in the structure that are important for selective binding of substrates/inhibitors/effectors;

  • the visualCMAT web-service to select and interpret correlated mutations/co-evolving residues in protein structures.

Implementation of Mustguseal, Zebra, pocketZebra, and visualCMAT in the laboratory practice can help at studying mechanisms of protein action, designing enzymes with improved properties for practical application and selective modulators of activity of the wild-type proteins. Find out more about the advanced tools to study the Mustguseal alignment.

Mustguseal support:

Dmitry Suplatov

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